Appearance (Colour)
Cream
Appearance (Form)
Free flowing, homogeneous powder
Solubility
37.00 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
pH (25°C)
7.4 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
clear to slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Cultural Response
Inoculate and incubate at 37°C for 18 - 24 hours
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Organism
Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100
Growth : Good
Organism
Enterococcus faecalis ATCC 29212
Inoculum (cfu) 10-100
Growth : Good
Applications
For the cultivation of variety of fastidious and non-fastidious aerobic and anaerobic microorganisms.
Composition
Ingredients
gm/lt.
Brain heart solids from infusion
250.00
Calf brain solids from infusion
250.00
Sodium chloride
5.00
Peptic digest of animal tissue
10.00
Dextrose
2.00
Disodium phosphate
2.50
Directions
1. Add 37.00 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Dispense into test tubes.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
