Appearance (Colour)
Yellow
Appearance (Form)
Free flowing, homogeneous powder
Solubility
29.00 gm/liter
Solubility before autoclaving (Clarity)
Clear without any precipitate
pH (25°C)
7.2 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear without any precipitate
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Cultural Response
Inoculate and incubate at 30- 35°C for upto 1 week
Organism
Clostridium perfringens ATCC 13124
Inoculum (cfu) 10-100
Growth : Good
H2S production : (+)
Organism
Clostridium sporogenes ATCC 11437
Inoculum (cfu) 10-100
Growth : Good
H2S production : (+)
Key
(+) : positive reaction, blackening of medium
Applications
For the cultivation of Clostridia from water samples.
Composition
Ingredients
gm/lt.
Veg. Peptone
10.00
Veg, extract
10.00
Sodium acetate, hydrated
5.00
Yeast extract
1.50
Starch
1.00
Dextrose
1.00
L-Cysteine hydrochloride
0.50
Directions
1. Add 29.00 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. To 25 ml of single strength medium, add 0.5 ml of filter sterilized solution prepared by mixing equal volumes of 4.0% Sodium sulphite and 7.0% Ferric citrate.
5. To 10 ml or 50 ml double strength medium, add 0.4 ml and 2 ml of the above solution.
