Appearance (Colour)
Off white
Appearance (Form)
Free flowing, homogeneous powder
Solubility
40.70 gm/liter
Solubility before autoclaving (Clarity)
Opalescent
Gel strength (1.5% gel)
Firm, comparable with 1.5% gel
pH (25°C)
7.4 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Whitish
Cultural Response
Inoculate and incubate at 37°C for 5 days
Organism
Azotobacter vinelandii ATCC 478
Inoculum (cfu) 10-100
Growth : Good
Organism
Azotobacter nigricans ATCC 35009
Inoculum (cfu) 10-100
Growth : Good
Applications
For the cultivation of Azotobacter species that can utilize glucose as carbon and atmospheric nitrogen as nitrogen source.
Composition
Ingredients
gm/lt.
Glucose
20.00
Dipotassium phosphate
0.20
Magnesium sulphate
0.20
Sodium chloride
0.20
Potassium sulphate
0.10
Calcium carbonate
5.00
Agar
15.00
Directions
1. Add 40.70 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
