Appearance (Colour)
Beige with pinkish tint
Appearance (Form)
Free flowing, homogeneous powder
Solubility
36.24 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength (1.5% gel)
Firm, comparable with 1.5% agar gel
pH (25°C)
7.5 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Clarity)
Greenish blue
Cultural Response
Inoculate and incubate at 37°C for 18 -24 hours
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate :>=70.0%
Colony colour : golden yellow
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate :>=70.0%
Colony colour : greyish green, mucoid
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate :>=70.0%
Colony colour : bright pink w/pink halo
Organism
Proteus mirabilis ATCC 25933
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate :>=70.0%
Colony colour : blue-green
Organism
Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate :>=70.0%
Colony colour : greyish green
Organism
Enterococcus faecalis ATCC 29212
Inoculum (cfu) 10-100
Growth : Good
Colony colour : orange - yellow or greenish
Applications
For differentiating various microorganisms depending on their colony characteristics.
Composition
Ingredients
gm/lt.
Peptone
4.00
Beef extract (ex. buffalo)
3.00
Agar
15.00
Casein enzymic hydrolysate
4.00
Lactose
10.00
L-Cystine
0.128
Bromothymol Blue
0.02
Andrade indicator
0.10
Directions
1. Add 36.248 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
