Appearance (Colour)
Biege
Appearance (Form)
Free flowing, homogeneous powder
Solubility
26.53 gm/liter
Solubility before autoclaving (Clarity)
Clear
pH (25°C)
7.4 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear
Prepared Medium Appearance after autoclaving (Colour)
Reddish purple
Cultural Response
Inoculate and incubate at 35 ± 2°C for 18-24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Inhibited
Applications
For detection and enumeration of coliforms from water and foods.
Composition
Ingredients
gm/lt.
Peptone
7.00
Yeast extract
3.00
Lactose
10.00
Sodium chloride
5.00
Bile salts mixture
1.50
Neutral Red
0.03
Crystal Violet
0.002
Directions
1. Add 26.53 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Do not autoclave.
