Appearance (Colour)
Brownish yellow, free flowing, homogeneous powder
Solubility (Colour) 2% aq. solution
Medium amber to yellowish brown, clear to slightly opalescent
pH (25°C)
5.00 - 6.50 (2%)
Stability (After autoclaving)
Medium amber to yellowish brown, clear to slightly opalescent
Loss on drying
6%
Nitrogen content (N)
9.0 - 11.0 %
Total Aerobic microbial count
(By plate count method) : <10, 000 cfu/gm
Total Yeast and Mould count
(By plate count method) : < 100 cfu/gm
Coagulable Protein Test
Passes test
Cultural Response
Growth Promotion Parameters :
Inoculate and incubate at 37° C for 18 - 48 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Indole Production (0.1%)
Escherichia coli ATCC 25922
Growth : Good, Result : Positive
Organism
Bacillus subtilis ATCC 6633
Organism
Growth : Good
Ash
11.0 - 16.0 %
NaCl
< 5.0 %
Applications
Low pH, slightly turbid, extract of autolysed yeast cells used generally for technical purposes.