Appearance (Colour)
Pink
Appearance (Form)
Free flowing, homogeneous powder
Solubility
59.03 gm/liter
Prepared Medium Appearance before autoclaving (Clarity)
Very slightly to slightly opalescent
Prepared Medium Appearance before autoclaving (Colour)
Red
Gel strength
Firm, comparable with 1.8% agar gel
pH (25°C)
7.4 ± 0.2
Prepared medium appearance after boiling (clarity)
Clear to very slightly opalescent
Prepared medium appearance after boiling (colour)
Red
Cultural Response
Inoculate and incubate at 35-37°C for 18 - 24 hours with added XLT4 Supplement
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Colony colour : Red with black centres
Organism
Salmonella enteritidis
ATCC 13076
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Colony colour : Red with black centres
Organism
Escherichia coli ATCC 25922
Inoculum
Growth : Partially inhibited
Recovery Rate : ~40.0%
Colony colour : Yellow
Organism
Staphylococcus aureus ATCC 25923
Inoculum
>=10³
Growth : Inhibited
Organism
Enterococcus faecalis ATCC 29212
Inoculum
>=10³
Growth : Inhibited
Organism
Proteus mirabilis ATCC 25933
Inoculum
>=10³
Growth : Inhibited
Applications
Used for isolation and identification of Salmonellae from various samples.
Composition
Ingredients
gms/lt.
Proteose peptone No. 3
1.60
Yeast extract
3.00
L-lysine
5.00
Xylose
3.75
Lactose
7.50
Saccharose
7.50
Ferric ammonium citrate
0.80
Sodium thiosulphate
6.80
Sodium chloride
5.00
Phenol Red
0.08
Agar
18.00
Directions
1. Add 59.03 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Add 4.60 ml XLT4 Supplement (16975).
3. Gently heat and bring to boiling.
4. Do not autoclave or overheat.
