Appearance (Colour)
Beige
Appearance (Form)
Free flowing, homogeneous powder
Solubility
41.53 gm/liter
Solubility before boiling (clarity)
Clear to slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
7.4 ± 0.2
Prepared medium appearance after boiling (clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Reddish purple
Cultural Response
Inoculate and incubate at 37°C for 18- 24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate: >=50.0%
Colony colour : Pink-red
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate: >=50.0%
Colony colour : Pink-red
Organism
Salmonella enteritidis ATCC 13076
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate: >=50.0%
Colony colour : Colourless
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Inhibited
Applications
Used for cultivation and isolation of Enterobacteriaceae in pharmaceutical testing according to harmonized methods.
Composition
Ingredients
gm/lt.
Pancreatic digest of gelatin
7.00
Yeast extract
3.00
Agar
15.00
Glucose
10.00
Sodium chloride
5.00
Bile salts
1.50
Neutral Red
0.03
Crystal Violet
0.002
Directions
1. Add 41.53 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Do not autoclave.
