Appearance (Colour)
Beige
Appearance (Form)
Free flowing, homogeneous powder
Solubility
37.10 gm/liter
Solubility before autoclaving (Clarity)
Slightly opalescent
Gel strength
Firm, comparable with 1.2% agar gel
pH (25°C)
7.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent w/o precipitate
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Cultural Response
Inoculate and incubate at 25°- 30°C for 3 -5 days
Organism
Aspergillus niger ATCC 16404
Inoculum (cfu) 10-100
Growth : Good
Colony colour : Light blue w/black spores
Recovery rate : -
Organism
Candida albicans ATCC 102321
Inoculum (cfu) 10-100
Growth : Good
Colony colour : Green
Recovery rate : >=50%
Organism
Saccharomyces cerevisiae ATCC 6633
Inoculum (cfu) 10-100
Growth : Good
Colony colour : Colourless
Recovery rate : >=50%
Organism
Escherichia coli ATCC 25922
Inoculum
Growth : Inhibited
Applications
Selective and differential medium for isolation of yeasts and moulds from milk and milk products.
Composition
Ingredients
gm/lt.
Yeast extract
4.00
Dextrose
20.00
Chromogenic mixture
1.10
Agar
12.00
Directions
1. Add 18.55 gm powder to distilled/purified water.
2. Bring volume to 500.0 ml and mix thoroughly.
3. Gently heat and bring to boiling.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
5. If desired, dispense aseptically contents of one vial of Oxytetra Selective Supplement. (51521) to 500.0 ml media.
6. Mix well and pour into sterile plates.
