Appearance (Colour)
Yellow powder
Cultural Response
Inoculate and incubate at 37 ± 2°C for 24-48 hours (Aseptically add rehydrated contents of 1 vial of Listeria Selective Supplement (PALCAM) (90345))
Organism
Listeria monocytogenes ATCC 19117
Inoculum (cfu) 10-100
Growth : Good
Colony characteristics : Grey green with black centre and black halo
Organism
Staphylococcus aureus ATCC 25923
Inoculum
Growth : None-poor
Colony characteristics : Yellow colonies with yellow halos
Organism
Enterococcus faecalis ATCC 29212
Inoculum
Growth : None-poor
Colony characteristics : Grey colonies with brown green halos
Applications
Used for the isolation of Listeria monocytogenes and other species of Listeria from food products.
Composition
Ingredients
Polymyxin B sulphate
5000 IU/ vial
Ceftazidime
10 mg/ vial
Acriflavine hydrochloride
2.500 mg/vial
Acriflavine hydrochloride
2.500 mg/ vial
Directions
1. Aseptically add 5.0 ml of sterile distilled water to 1.0 vial.
2. Mix well and aseptically add to sterile cooled media at 45-50°C and pour in petri plates.
3. Add 5.0 ml (1 vial) to 500 ml media -
Listeria Identification Agar Base (PALCAM) (96310)
