Appearance (Form)
Free flowing, homogeneous powder
Solubility
44.56 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
pH (25°C)
6.8 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Purple
Cultural Response
Inoculate and incubate at 25-30° C for 24 - 48 hours
Organism
Klebsiella pneumoniae ATCC 13883
Inoculum (cfu) 10-100
Growth : Good
Slant : Alkaline,purple color
Butt : Acid,yellowing of medium
H2S : (- ve) No blackening of medium
Gas : ( + ve)
Organism
Yersinia enterocolitica ATCC 27729
Inoculum (cfu) 10-100
Growth : Good
Slant : Alkaline,purple color
Butt : Acid,yellowing of medium
H2S : (- ve) No blackening of medium
Gas : ( - ve)
Applications
For the cultivation and differentiation of bacteria based on their ability to decarboxylate lysine, arginine and produce H2S.Recommended by APHA for isolation and identification of Yersinia from milk and milk products.
Composition
Ingredients
gms/lt.
L-lysine
10.00
Peptic digest of animal tissue
5.00
Yeast extract
3.00
L-Arginine
10.00
Dextrose
1.00
Ferric ammonium citrate
0.50
Sodium thiosulphate
0.04
Bromocresol Purple
0.02
Agar
15.00
Directions
1. Add 44.56 gm powder to distilled/purified water.
2. Bring volume to 1.0 litre and mix thoroughly.
3. Gently heat and bring to boiling.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
5. Dispense as desired, preferably in slants with deep butts.
