Appearance (Colour)
Yellow
Appearance (Form)
Free flowing, homogeneous powder
Solubility
40.00 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength (1.5% gel)
Firm, comparable with 1.5% agar gel
pH (25°C)
7.3 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light amber (Note : After addition of 5% sterile defibrinated sheep blood: Cherry red, opaque.)
Cultural Response
Inoculate and incubate at 37°C for 18- 24 hours
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth w/o blood : Good
Recovery Rate : >=70%
Growth w/blood: Good
Recovery Rate : >=70%
Haemolysis : Beta
Organism
Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100
Growth w/o blood : Fair-Good
Recovery Rate : ~50%
Growth w/blood: Good
Recovery Rate : >=70%
Haemolysis : Beta
Organism
Staphylococcus epidermidis ATCC 12228
Inoculum (cfu) 10-100
Growth wo/blood: Fair-Good
Recovery Rate : ~50%
Growth w/blood: Good
Recovery Rate : >=70%
Haemolysis : None
Organism
Streptococcus pneumoniae ATCC 6303
Inoculum (cfu) 10-100
Growth wo/blood: Fair-Good
Recovery Rate : ~50%
Growth w/blood: Good
Recovery Rate : >=70%
Haemolysis : Alpha
Applications
For the isolation, cultivation and detection of haemolytic activity of streptococci and other fastidious microorganisms.
Composition
Ingredients
gms/lt.
Agar
15.00
Sodium chloride
5.00
Beef extract
10.00
Peptone
10.00
Directions
1. Add 40.00 gm powder to distilled/purified water.
2. Bring volume to 950 ml and mix thoroughly.
3. Gently heat and bring to boiling.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
5. Cool to 45-50°C.
6. Add aseptically 50.0 ml sterile defibrinated sheep blood, mix thoroughly.
7. Dispense into sterile petri plates.
