Appearance of Solution
Liquid
Appearance (Colour)
Colourless
Appearance (Clarity)
Clear
Coating enzyme
Apyrase/RNAse/Alkaline phosphatase/ Diaphorase
Coating antigen
ELIZA with His-tag Antigen
pH
8.5
Description
Coating buffers are generally used for adsorptive immobilization of proteins and antibodies on plastic surfaces like microtiter or ELISA plates or protein - binding plastic/ glass surfaces.The coating buffers play a major role in defining the efficiency/ sensitivity of an assay or diagnostic test. Majority of commercial buffers use Phosphate buffer saline (PBS) and Carbonate buffer which requires 1 hour to overnight incubation to coat proteins / antibodies. Express coating buffer is used for rapid adsorptive immobilization of proteins /enzymes/antibodies on any plastic surfaces including ELISA plates.
