Appearance (Colour)
White, fine powder
Solubility
Soluble in distilled water
Cultural Response
Inoculate and incubate at 35 ± 2°C for 18-24 hours under anaerobic conditions (Aseptically,add the rehydrated contents of 1 vial of Perfringens S.F.P. Supplement (92096) as per direction)
Organism
Clostridium perfringens ATCC 12924
Inoculum (cfu) 10-100
Growth : Good
Sulphite reduction : Positive
Organism
Escherichia coli ATCC 25922
Inoculum
Growth : Inhibited
Applications
Used for the selective isolation of clostridium perfringens.
Composition
Kanamycin Sulphate
6.00 mg/vial
Polymixin B
15,000 IU/vial
Directions
1. Aseptically add 2 ml of sterile distilled water to 1 vial.
2. Mix well and aseptically add to sterile cooled media at 45-50°C and pour in petriplates.
3. Add 2 ml (1 vial) of Perfringens S.F.P. supplement to 475 ml of Perfringens Agar Base (T.S.C/S.F.P.) or S.F.P. Agar Base (11580) along with 25 ml of egg yolk emulsion.
