Appearance (Colour)
Light tan
Appearance (Form)
Free flowing, homogeneous powder
Solubility
50.00 gm/liter
Solubility before autoclaving (Clarity)
Opalescent
Gel strength
Firm, comparable with 0.75% agar gel and 1.0%Gelatin gel.
pH (25°C)
7.5 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Opalescent
Prepared Medium Appearance after autoclaving (Colour)
Medium amber
Cultural Response
Inoculate and incubate at 35-37°C for 18-48 hours
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth- good
Organism
Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100
Growth- good
Organism
Streptococcus pneumoniae ATCC 6305
Inoculum (cfu) 10-100
Growth- good
Applications
Used for maintaining stock cultures of bacteria, particularly streptococci.
Composition
Ingredients
gm/lt.
Beef heart, infusion from (ex.buffalo)
500.00
Proteose peptone
10.00
Gelatin
10.00
Dextrose
0.50
Iso electric Casein
5.00
Disodium phosphate
4.00
Sodium citrate
3.00
Agar
7.50
Directions
1. Add 50.00 gm powder to distilled/purified water.
2. Bring volume to 1.0 liter and mix thoroughly.
3. Gently heat and bring to boiling.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
