Appearance (Form)
Liquid
Appearance (Colour)
Colouless
Appearance (Clarity)
Clear
A260/A280
1.80-2.00
Agarose gel electrophoresis
Predominantly supercoiled Plasmid DNA (80-90%), relaxed plasmid DNA with no RNA seen
Description
pUC18 is a high copy number plasmids derived from pBR322. This 2686 bp long plasmid contains an ampicillin resistance-conferring gene. pUC18 and pUC19 are identical except that they contain multiple cloning sites (MCS) arranged in opposite orientations. It is a popular teaching aid for demonstrating plasmid DNA isolation, restriction digestion and cloning.
