Appearance (Colour)
Light pink
Appearance (Form)
Free flowing, homogeneous powder
Solubility
24.81 gm/liter
Solubility before autoclaving (Clarity)
Very slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
6.9 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Orange red
Cultural Response
Inoculate and incubate at 35 ± 2°C for 24-48
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth- Good, Colour of the slant- Cerise
Organism
Salmonella serotype Typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth- Good, Colour of the slant- Cerise
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth- Good, Colour of the slant- No change
Organism
Salmonella serotype Enteritidis ATCC 13076
Inoculum (cfu) 10-100
Growth- Good, Colour of the slant- Cerise
Organism
Klebsiella pneumoniae ATCC 13883
Inoculum (cfu) 10-100
Growth- Good, Colour of the slant- Orange to pink
Applications
For the differentiation of enteric pathogens based on their ability to utilize citrate as a carbon source.
Composition
Ingredients
gm/lt.
Yeast extract
0.50
L-Cysteine hydrochloride
0.10
Sodium citrate
3.00
Dextrose
0.20
Monopotassium phosphate
1.00
Sodium chloride
5.00
Phenol Red
0.012
Agar
15.00
Directions
1. Add 24.81 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Dispense into test tubes.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
