Appearance (Colour)
White, fine powder
Solubility
Soluble in distilled water
Cultural Response
Inoculate and incubate at 35 ± 2°C for 48 hours. (Aseptically add the rehydrated contents of 1 vial as per direction) Growth observed on Clostridium Difficile Agar Base
Organism
Clostridium difficile ATCC 11204
Inoculum (cfu) 10-100
Growth : Good
Colony colour : Greyish white
Organism
Shigella flexneri ATCC 12022
Inoculum
Growth : Inhibited
Organism
Escherichia coli ATCC 25922
Inoculum
Growth : Inhibited
Organism
Staphylococcus aureus ATCC 25923
Inoculum
Growth : Inhibited
Applications
Used for the selective isolation of Clostridium difficile.
Composition
D-Cycloserine
250.00 mg/vial
Cefoxitin
8.00 mg/vial
Directions
1. Aseptically add 2ml of sterile distilled water to 1 vial.
2. Mix well and aseptically add to sterile cooled media at 45-50°C and pour in pertriplates.
3. Add 2ml (1 vial) of supplement to 500 ml of Clostridium Difficile Agar Base.
