Appearance (Colour)
Cream
Appearance (Form)
Free flowing, homogeneous powder
Solubility
46.70 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength (1.5% gel)
Firm, comparable with 1.5% agar gel
pH (25°C)
7.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Cultural Response
Inoculate and incubate at 37°C for 18 -24 hours
Organism
Pseudomonas aeruginosa ATCC 9027
Inoculum (cfu) 10-100
Growth : Good, Recovery rate : >=50%
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Good, Recovery rate : >=50%
Organism
Escherichia coli ATCC 25922
Inoculum
Growth : Inhibited
Organism
Staphylococcus aureus ATCC 25923
Inoculum
Growth : Inhibited
Applications
Made from Veg.based ingredients ; it is used for the selective cultivation and identification of Pseudomonas aeruginosa.
Composition
Ingredients
gm/lt.
Veg . Peptone
20.00
Magnesium chloride
1.40
Potassium sulphate
10.00
Cetrimide
0.30
Agar
15.00
Directions
1. Add 46.70 gm powder to 1.0 liter distilled/purified water, containing 10.0 ml glycerol.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. If desired,add rehydrated contents of 1 vial of Nalidixic Selective supplement (67124).
