Appearance (Colour)
Cream
Appearance (Form)
Free flowing, homogeneous powder
Solubility
31.50 gm/liter
Solubility before autoclaving (Clarity)
Clear solution
pH (25°C)
7.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear solution
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Cultural Response
Inoculate and incubate at 37°C for 18 - 24 hours
Organism
Shigella dysenteriae ATCC 13313
Inoculum (cfu) 10-100
Growth : Good
Organism
Shigella flexneri ATCC 12022
Inoculum (cfu) 10-100
Growth : Good
Organism
Shigella sonnei ATCC 25931
Inoculum (cfu) 10-100
Growth : Good
Organism
Staphylococcus aureus ATCC 25923
Inoculum
Growth : Inhibited
Applications
Used for the isolation and cultivation of Shigella species from food.
Composition
Ingredients
gm/lt.
Enzymatic digest of casein
20.00
Dextrose
1.00
Sodium chloride
5.00
Dipotassium hydrogen phosphate
2.00
Potassium dihydrogen orthophosphate
2.00
Polysorbate 80
1.50
Directions
1. Add 31.50 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Cool to 45-50°C and aseptically add 1 vial of Shigella selective supplement (45290). Mix well.
