Appearance (Form)
Liquid
Appearance (Colour)
Colourless
Absence of nucleases
No RNase, DNase contamination detected
Description
RT-PCR Kit is optimized to synthesize first-strand cDNA from purified poly (A)+ or total RNA. The RT-PCR kit for RT-PCR delivers increased cDNA yields, high sensitivity, and full-length transcripts in a convenient format. All the components needed for successful first-strand cDNA synthesis is included, saving time and ensuring success with every experiment. The kit Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability.
The enzyme is used to synthesize cDNA at a
temperature range of 42-55°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases.
Applications
First strand cDNA synthesis for RT-PCR.
Construction of cDNA libraries.
One-step RT-PCR
Primer extension
Includes
20 preps
· M-MLV (Recombinant) (200U/μl) - 20.0 μl
· Reaction Buffer (10X) - 40.0 μl
· Oligo (dT) 15 Primer (50μM) - 20.0 μl
· Random Hexamer Primer 6-mer (50μM) - 20.0 μl
· RNasin (40U/ μl) - 12.5 μl
· dNTP Mix (10mM each) - 50.0 μl
· RNase Free Water - 1.0 ml
40 preps
· M-MLV (Recombinant) (200U/μl) - 40.0 μl
· Reaction Buffer (10X) - 80.0 μl
· Oligo (dT) 15 Primer (50μM) - 40.0 μl
· Random Hexamer Primer 6-mer (50μM) - 40.0 μl
· RNasin (40U/ μl) - 25.0 μl
· dNTP Mix (10mM each) - 100.0 μl
· RNase Free Water - 2.0 ml
100 preps
· M-MLV (Recombinant) (200U/μl) - 100.0 μl
· Reaction Buffer (10X) - 200.0 μl
· Oligo (dT) 15 Primer (50μM) - 100.0 μl
· Random Hexamer Primer 6-mer (50μM) - 100.0 μl
· RNasin (40U/ μl) - 60.0 μl
· dNTP Mix (10mM each) - 250.0 μl
· RNase Free Water - 5.0 ml
