Appearance (Colour)
Brown
Appearance (Form)
Powder
Activity
min. 800 units/mg powder
Unit Definition
One unit will cause an increase in A280nm of 0.001 per minute at pH 6.5 at 25°C in a 3.0 ml reaction mix containing L-tyrosine.
Enzyme solution for activity determination
Prepared in potassium phosphate buffer, pH 6.5.
Application
Converts tyrosine to L-DOPA or melamine in mammals or tyramine to dopamine. The enzyme from mushrooms has a molecular weight of 128,000 and is frequently used in Browning reactions.