Activity
Lambda DNA Digests of the teaching kit were ligated using T4DNA ligase after incubating at 16°C for one hour
Description
The overall goal is to join linear DNA fragments together with covalent bonds. More specifically, DNA ligation involves creating a phosphodiester bond between the 3' hydroxyl of one nucleotide and the 5' phosphate of another. The term recombinant DNA encapsulates the concept of recombining fragments of DNA from different sources into a new and useful DNA molecule. This ligation kit contains material for ligation of two lambda DNA digests using the enzyme T4 DNA ligase.
Includes
Lambda Digest1 (250ng/mcl) - 80.0 mcl
Lambda Digest2 (250ng/mcl) - 80.0 mcl
10 X Reaction buffer - 100 mcl
T4 DNA Ligase (20 units/mcl) - 15 mcl
Sterile Water - 500 mcl
6 X Gel loading Dye - 200 mcl
Agarose - 1.2 gm
Ethidium Bromide - 30 mcl
50 X TAE buffer - 25 ml
DNA marker - 60 mcl
