Appearance (Colour)
Biege
Appearance (Form)
Free flowing, homogeneous powder
Solubility
41.10 gm/liter
Solubility before autoclaving (Clarity)
Very slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
6.2 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Medium blue
Cultural Response
Inoculate and incubate at 25 - 30°C for 24-48 hours
Organism
Candida albicans ATCC 10231
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate :>=70%, Fluorescence : +
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate :>=70%, Fluorescence : -
Organism
Bacillus subtilis ATCC 6633
Inoculum (cfu) 10-100
Growth : Poor, Recovery Rate :>=70%,Fluorescence : -
Key
Fluorescence : + indicates yellow-green fluorescence
Fluorescence : - indicates no fluorescence
Applications
For the isolation and differentiation of Candida albicans.
Composition
Ingredients
gm/lt.
Peptone
5.00
Agar
20.00
Glucose
10.00
Yeast extract
3.00
Malt Extract
3.00
Aniline Blue
0.10
Directions
1. Add 41.10 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Bring volume to 1.0 litre and mix thoroughly.
3. Gently heat and bring to boiling.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
