Appearance (Colour)
Light to medium tan
Appearance (Form)
Free flowing,homogeneous powder
Solubility (Colour) 2% aq. solution
Light to medium amber, clear
pH (25°C)
7.0 ± 0.5 (2%)
Stability (After autoclaving)
Light to medium amber, clear
Loss on drying
< 5.0 %
Nitrogen content (N)
11.5 - 15.5 %
NaCl
< 5.0 %
Ash
< 10.0 %
Total Aerobic microbial count
(By plate count method) : < = 10,000 cfu/gm
Total Yeast and Mould count
(By plate count method) : <=100 cfu/gm
Coagulable Protein Test
Passes Test
Proteose test
Passes Test
Nitrite test
Passes Test
Cultural Response
Growth Promotion Properties :
Inoculate and incubate at 37°C for 18- 24 hours
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Good
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good
Indole Production (0.1%)
Escherichia coli ATCC 25922
Growth : Good, Result : Positive
Fermentable carbohydrates (2%)
Escherichia coli ATCC 25922
Growth : Good, Result : Negative
Hydrogen Sulphide Production (1.0%)
Salmonella typhimurium ATCC 14028
Growth : Good, Result : Positive
Acetylmethylcarbinol Production
Enterobacter aerogenes ATCC 13048
Growth: Good, Result : Positive
Applications
Used in various general and diagnostic media, also for large-scale production of enzymes, vaccines, antibiotics, etc. Peptic digest of animal tissue also used in microbiological culture media.
