Appearance (Colour)
Pinkish purple
Appearance (Form)
Free flowing, homogeneous powder
Solubility
35.96 gm/liter
Solubility before autoclaving (Clarity)
Opalescent, with a uniform precipitate
Gel strength (1.5% gel)
Firm, comparable with 1.5% agar gel
pH (25°C)
7.2 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Opalescent, with finely disperesed precipitate
Prepared Medium Appearance after autoclaving (Colour)
Purple w/green orange cast
Cultural Response
Inoculate and incubate at 37°C for 18 - 24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >= 50.0%
Colony colour : Blue- black w/dark centers with green metallic sheen
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >= 50.0%
Colony colour : Pink without sheen
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >= 50.0%
Colony colour : Colourless
Organism
Klebsiella pneumoniae ATCC 13883
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >= 50.0%
Colony colour : Pink mucoid
Organism
Proteus mirabilis ATCC 25933
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >= 50.0%
Colony colour : Colourless
Organism
Enterococcus faecalis ATCC 29212
Inoculum (cfu) 10-100
Growth : Partially inhibited
Colony colour : Colourless
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Inhibited
Applications
For the isolation, cultivation and differentiation of gram negative enteric bacteria based on lactose fermentation.
Composition
Ingredients
gm/lt.
Peptone
10.00
Agar
13.50
Lactose
5.00
Sucrose
5.00
Dipotassium hydrogen phosphate
2.00
Eosin Y
0.40
Methylene Blue
0.065
Directions
1. Add 35.96 gm powder to distilled/purified water.
2. Bring volume to 1.0 liter and mix thoroughly.
3. Gently heat and bring to boiling.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
