Appearance (Colour)
White
Appearance (Form)
Free flowing, homogeneous powder
Solubility
14.50 gm/liter
Solubility before autoclaving (Clarity)
Clear solution without any precipitate
pH (25°C)
7.4 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear solution without any precipitate
Prepared Medium Appearance after autoclaving (Colour)
Colourless
Cultural Response
Inoculate and incubate at 37°C for 18-24 hours
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Good w/greenish yellow pigment
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Fair-poor
Organism
Pseudomonas aeruginosa ATCC 9027
Inoculum (cfu) 10-100
Growth : Good w/greenish yellow pigment
Applications
For cultivation of Pseudomonas aeruginosa by membrane filter technique.
Composition
Ingredients
gm/lt.
DL- Asparagine
2.00
L-Proline
1.00
Dipotassium phosphate, anhydrous
1.00
Magnesium sulphate
0.50
Potassium sulphate
10.00
Directions
1. Add 14.50 gm powder to 1.0 liter distilled/purified water containing 25.0 ml ethanol and mix thoroughly.
2. Gently heat and bring to boiling.
3. Dispense into test tubes.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes
