Appearance (Colour)
Beige
Appearance (Form)
Free flowing, homogeneous powder
Solubility
23.00 gm/lit
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength
Firm, comparable with 1.5% Agar gel
pH (25°C)
7.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light to medium amber
Cultural Response
Inoculate and incubate at 35 ± 2°C for 18-24 hours with the following:
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth: Good
Recovery Rate :>=70.0%
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth: Good
Recovery Rate :>=70.0%
Organism
Enterococcus faecalis ATCC 29212
Inoculum (cfu) 10-100
Growth: Good
Recovery Rate :>=70.0%
Organism
Staphylococcus aureus ATCC 25923
Inoculum
Growth: Good
Recovery Rate :>=70.0%
Organism
Shigella flexneri ATCC 12022
Inoculum (cfu) 10-100
Growth: Good
Recovery Rate :>=70.0%
Organism
Salmonella typhi ATCC 6539
Inoculum (cfu) 10-100
Growth: Good
Recovery Rate :>=70.0%
Organism
Salmonella enteritidis ATCC 13076
Inoculum (cfu) 10-100
Growth: Good
Recovery Rate :>=70.0%
Applications
For the cultivation of a wide variety of microorganisms,including Salmonella species
Composition
Ingredients
gms/lt.
Agar
15.00
Peptic digest of animal tissue
5.00
Beef extract (ex.buffalo)
3.00
Directions
1. Add 23.00 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Cool to 45-50°C and if desired,add 5-10% v/v sterile defibrinated blood.Mix well.
