Appearance (Colour)
Yellow
Appearance (Form)
Free flowing, homogeneous powder
Solubility
9.00 gm/liter
Solubility before autoclaving (Clarity)
Clear without any precipitate
pH (25°C)
6.8 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear without any precipitate
Prepared Medium Appearance after autoclaving (Colour)
Purple
Cultural Response
Inoculate and incubate at 37°C for 4 days
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Lysine : (±)ve
Arginine : (±)ve
Ornithine : (±)ve
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good
Lysine : (+)ve
Arginine : (-)ve
Ornithine : (+)ve
Organism
Salmonella serotype Typhi ATCC 6539
Inoculum (cfu) 10-100
Growth : Good
Lysine : (+) ve
Arginine : (+)ve or (-)ve
Ornithine : (+)ve
Organism
Klebsiella pneumoniae ATCC 13883
Inoculum (cfu) 10-100
Growth : Good
Lysine : (+)ve
Arginine : (-)ve
Ornithine : (-)ve
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Good
Lysine : (-)ve
Arginine : (+)ve
Ornithine :(-)ve
Organism
Shigella flexneri ATCC 12022
Inoculum (cfu) 10-100
Growth : Good
Lysine :( -)ve
Arginine : (+)ve or (-)ve
Ornithine : (-)ve
Key
(±)ve : variable reaction
(+)ve : positive reaction,purple colour
(-)ve : negative reaction,yellow colour
Applications
For the differentiation of bacteria based on their ability to decarboxylate the amnio acid.
Composition
Ingredients
gm/lt.
Peptone
5.00
Yeast extract
3.00
Dextrose
1.00
Bromocresol Purple
0.02
Directions
1. Add 9.00 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Distribute the medium into 4 equal parts into test tubes.
4. In the first test tube no amino acid is to be added.
5. To the remaining 3 parts, add separately 3 L-amino acids, L-Lysine hydrochloride, L-Arginine hydrochloride and L-Ornithine hydrochloride to a final concentration of 0.5%.
6. Dispense in 3 -4 ml quantities in screw capped tubes.
7. Autoclave at 15 psi pressure at 121°C for 15 minutes.
Note : To avoid false alkalinization at the surface of medium ;add liquid paraffin to a height of about 5mm
before sterilization.
