Appearance (Form)
Liquid
Appearance (Colour)
Colourless
Appearance (Clarity)
Clear
DNase activity
None
Protease activity
None
Description
RNase A Solution is DNase and protease-free. It is an endoribonuclease extracted from bovine pancreas that specifically degrades single-stranded RNA. It cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide. The resulting 2', 3'-cyclic phosphate is hydrolyzed to the corresponding 3'-nucleoside phosphate.It is a monomer of 13.7kDa. It is DNase Free and requires no heating prior to use.
Applications
Plasmid and genomic DNA preparation.
Removal of RNA from recombinant protein preparations. Ribonuclease protection assays
Mapping single-base mutations in DNA or RNA
Includes
Ribonuclease A Solution (10mg/ml)
