Appearance (Colour)
Pinkish biege
Appearance (Form)
Free flowing, homogeneous powder
Solubility
51.50 gm/liter
Solubility before autoclaving (Clarity)
Very slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
7.1 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Reddish purple
Cultural Response
Inoculate and incubate at 37° C for 18 - 24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Recovery : >=50.0%
Colony colour : Pink w/bile precipitate
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good
Recovery : >=50.0%
Colony colour : Pink to red
Organism
Klebsiella pneumoniae ATCC 13883
Inoculum (cfu) 10-100
Growth : Good
Recovery : >=50.0%
Colony colour : Pink mucoid
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Good
Recovery : >=50.0%
Colony colour : colourless
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Good
Recovery : >=50.0%
Colony colour : colourless
Organism
Proteus vulgaris ATCC 13315
Inoculum (cfu) 10-100
Growth : Good
Recovery : >=50.0%
Colony colour : colourless
Organism
Proteus mirabilis ATCC 12453
Inoculum (cfu) 10-100
Growth : Good
Recovery : >=50.0%
Colony colour : colourless
Organism
Staphylococcus aureus ATCC 25923
Inoculum
Growth : Inhibited
Organism
Salmonella Paratyphi B
Inoculum (cfu) 10-100
Growth : Good
Recovery : >=50.0%
Colony colour : colourless
Organism
Shigella flexneri ATCC 12022
Inoculum (cfu) 10-100
Growth : Good
Recovery : >=40.0%
Colony colour : colourless
Applications
For the selective isolation, cultivation and differentiation of coliforms and enteric pathogens based on ability to ferment lactose.
Composition
Ingredients
gm/lt.
Veg. Peptone
17.00
Agar
15.00
Lactose
10.00
Sodium chloride
5.00
Synthetic detergent
1.50
Veg. Hydrolysate
3.00
Neutral Red
0.03
Crystal Violet
0.001
Directions
1. Add 51.50 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
