Appearance (Colour)
Cream
Appearance (Form)
Free flowing, homogeneous powder
Solubility
13.00 gm/liter
Solubility before autoclaving (Clarity)
Clear
pH (25°C)
6.9 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear
Prepared Medium Appearance after autoclaving (Colour)
Light to medium amber, clear without any precipitate
Cultural Response
Inoculate and incubate at 37°C for 18 - 24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good, Gas Production : (+)
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good, Gas Production : (+)
Organism
Salmonella typhi ATCC 6539
Inoculum (cfu) 10-100
Growth : Good, Gas Production : (-)
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Good, Gas Production : (-)
Organism
Enterococcus faecalis ATCC 29212
Inoculum (cfu) 10-100
Growth : Good, Gas Production : (-)
Key
(+) : Positive Gas Production
(-) : Negative Gas Production
Applications
For the detection of lactose fermenting gram-negative coliforms, as pre-enrichment broth for Salmonella species.
Composition
Ingredients
gm/lt.
Peptone
5.00
Lactose
5.00
Beef extract (ex.buffalo)
3.00
Directions
1. Add 13.00 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Dispense into tubes containing inverted Durham’s tubes.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
