Appearance (Colour)
Light orange
Appearance (Form)
Free flowing, homogeneous powder
Solubility
9.00 gm/liter
Solubility before autoclaving (Clarity)
Clear without any precipitate
pH (25°C)
6.8 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear without any precipitate
Prepared Medium Appearance after autoclaving (Colour)
Yellowish-orange
Cultural Response
Inoculate and incubate at 37°C for 18-24 hours
Organism
Proteus vulgaris ATCC 13315
Inoculum (cfu) 10-100
Growth : Good
Urease production : (+)ve
Organism
Proteus mirabilis ATCC 25933
Inoculum (cfu) 10-100
Growth : Good
Urease production : (+)ve
Organism
Klebsiella pneumoniae ATCC 13883
Inoculum (cfu) 10-100
Growth : Good
Urease production :(+)ve
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Good
Urease production : (-)ve
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Urease production : (-)ve
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good
Urease production : (-)ve
Key
(+)ve Positive, pink-red colour
(-)ve Negative,no change in colour
Applications
For identifying bacteria on the basis of urea utilization, for the differentiation of Proteus species from Salmonella and Shigella species.
Composition
Ingredients
gms/lt.
Peptone
1.00
Dextrose
1.00
Sodium chloride
5.00
Disodium hydrogen phosphate
1.20
Monopotassium phosphate
0.80
Phenol Red
0.012
Directions
1. Add 9.00 gm powder to 950.0 ml of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Cool to 50°C.
5. Under aseptic conditions, add 50.0ml of sterile 40.0% Urea solution (80192) and mix well.
6. Dispense into sterile test tubes.
