Appearance (Colour)
Beige
Appearance (Form)
Free flowing, homogeneous powder
Solubility
67.52 gm/liter
Solubility before boiling (clarity)
Clear to slightly opalescent
Gel strength (1.5% gel)
Firm, comparable with 1.5% agar gel
pH (25°C)
7.2 ± 0.2
Prepared medium appearance after boiling (clarity)
Clear to slightly opalescent
Prepared medium appearance after boiling (colour)
Purple coloured
Cultural Response
Inoculate and incubate at 35-37°C for 18-24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : None-poor
Recovery rate : <=10.0%
Colour of colony : yellow
Organism
Proteus mirabilis ATCC 12453
Inoculum (cfu) 10-100
Growth : None-poor
Recovery rate : <=10.0%
Colour of colony : colourless
Organism
Salmonella enteritidis ATCC 13076
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : <=50.0%
Colour of colony : colourless may show faint bluish coloured colonies
Organism
Shigella dysenteriae ATCC 13313
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : <=50.0%
Colour of colony : colourless may show faint bluish coloured colonies
Organism
Shigella flexneri ATCC 12022
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : <=50.0%
Colour of colony : colourless may show faint bluish coloured colonies
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : <=50.0%
Colour of colony : colourless may show faint bluish coloured colonies
Organism
Shigella sonnei ATCC 25931
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : <=50.0%
Colour of colony : colourless may show faint bluish coloured colonies
Applications
Used for the selective isolation of Salmonella and Shigella species.
Composition
Ingredients
gm/lt.
Peptic digest of animal tissue
5.00
Yeast extract
3.00
Beef extract(ex.buffalo)
3.00
Pancreatic digest of casein
5.00
Lactose
7.50
Sucrose
7.50
Sodium citrate
10.00
Sodium chloride
5.00
Sodium thiosulphate
5.00
Sodium deoxycholate
2.50
Bromocresol Purple
0.02
Agar
14.00
Directions
1. Add 67.52 gm powder to 1.0 litre distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Cool to 45- 50°C.
4. Do not autoclave.
