Appearance (Colour)
Light yellow
Appearance (Form)
Free flowing, homogeneous powder
Solubility
44.00 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
7.3 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Opaque
Prepared Medium Appearance after autoclaving (Colour)
Before addition of blood : Light amber
After addition of blood : Cherry red
Cultural Response
Inoculate and incubate at 37°C for 24 - 48 hours
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Haemolysis : beta
Organism
Staphylococcus epidermidis ATCC 12228
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Haemolysis : gamma
Organism
Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Haemolysis : beta
Organism
Escherichia coli ATCC 25922
Inoculum
>=103,Growth : inhibited
Applications
For selective isolation and cultivation of pathogenic Gram-positive cocci from clinical and non-clinical specimens.
Composition
Ingredients
gm/lt.
Agar
15.00
Sodium chloride
5.00
Corn Starch
1.00
Biopeptone
20.00
Tryptose
3.00
Colistin sulphate
0.010
Nalidixic acid
0.015
Directions
1. Add 44.00 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Cool to 45 - 50°C.
5. Add aseptically 5.0% to 10.0% sterile defibrinated blood.
6. Mix well.
