Appearance of Solution
Liquid
Appearance (Colour)
Colourless
Appearance (Clarity)
Clear
Absence of nucleases
Free of endonuclease, exonuclease, RNase and E.coli RNA polymerase activities
Assay
Functional assay with high yield in T7 RNA transcription
Purity
>95% by SDS PAGE
Description
T7 RNA polymerase is a monomeric bacteriophage encoded DNA directed RNA polymerase which catalyzes the formation of RNA in the 5`-3` direction. In the process of initiation of transcription T7 recognizes a specific promoter sequence, the T7 promoter. T7 consists of 883 amino acids and has a molecular weight of 99 kDa.
Application
Radiolabeled RNA probe preparation
RNA generation for in vitro translation
RNA generation for studies of RNA structure, processing and catalysis
Expression control via anti-sense RNA
Vaccine production
In situ hybridization
Unit Definition:
One unit is defined as the amount of enzyme required to catalyze the incorporation of 5nmol of rCTP into acid-insoluble product in 1 hour at 37°C in a total volume of 100μl.
