Appearance (Colour)
Pinkish beige
Appearance (Form)
Free flowing, homogeneous powder
Solubility
50.00 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
7.1 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Purplish red
Cultural Response
Inoculate and incubate at 370C for 18-24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Colony colour : pink w/bile precipitate
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good
Colony colour : pink to red
Organism
Escherichia coli O157:H7 NCTC 29900
Inoculum (cfu) 10-100
Growth : Good
Colony colour : colourless
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Fair to Good
Colony colour : colourless
Applications
For the isolation and identification of pathogenic Escherichia coli.
Composition
Ingredients
gm/lt.
Peptic digest of animal tissue
17.00
Proteose peptone
3.00
Sorbitol
10.00
Bile salts
1.50
Sodium chloride
5.00
Neutral Red
0.03
Crystal Violet
0.001
Agar
15.30
Directions
1. Add 50.00 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
