Appearance (Colour)
Light yellow to Light brown
Appearance (Form)
Free flowing,homogeneous powder
Solubility (Colour) 2% aq. solution
Yellow, Clear, completely soluble
pH (25°C)
6.0 - 7.0 (2.0%)
Stability (After autoclaving)
Yellow, clear without any precipitate or scum on the surface of the solution
Loss on drying
< 5.0 %
Deoxycholic acid-sodium salt
~ 45.0 % - 50.0%
Total Aerobic microbial count
(By plate count method) : <10,000 cfu/gm
Escherichia coli ATCC 25922
Absent
Salmonella Typhimurium ATCC 14028
Absent
Staphylococcus Aureus ATCC 25923
Absent
Total Yeast and Mould count
(By plate count method) : <100 cfu/gm
Cultural Response
Prepare Brilliant Green Bile Broth, using Ox bile as ingredient. Inoculate and incubate at 37°C for 18-24 hours
Organism
Escherichia coli
ATCC 25922
Inoculum (cfu) 10-100
Growth : Good w/ Gas Production
Organism
Enterobacter aerogenes
ATCC 13048
Inoculum (cfu) 10-100
Growth : Good w/Gas Production
Organism
Staphylococcus aureus
ATCC 25923
Inoculum (cfu) 10-100
Growth : Inhibited
Applications
Dehydrated ox bile, used is preparation of microbiological culture media as a selectively inhibitory agent.It inhibits bacteria not growing in enteric environment and favours growth of enteric microorganisms.
