Appearance (Colour)
Colourless, clear solution
Solubility
3.5 %, Clear solution
Cultural Response
Inoculate and incubate at 35 ± 2°C for 24-48 hours (Aseptically,add the contents of 1 vial of Potassium tellurite 3.5% supplement (99482) as per direction) Growth observed on Baird-Parker Agar Base w/Sulpha (21748)
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Colony colour : Grey-black, shiny
Lecithinase halo : Positive
Organism
Staphylococcus aureus ATCC 6538
Inoculum (cfu) 10-100
Growth : Good
Colony colour : Black
Lecithinase halo : Positive
Organism
Proteus mirabilis ATCC 25933
Inoculum (cfu) 10-100
Growth : Good
Colony colour : Brown-black
Lecithinase halo : Negative
Organism
Staphylococcus epidermidis ATCC 12228
Inoculum (cfu) 10-100
Growth : Poor to good
Colony colour : Black
Lecithinase halo : Negative
Organism
Bacillus subtilis ATCC 6633
Inoculum (cfu) 10-100
Growth : None to poor
Colony colour : Brown
Lecithinase halo : Negative
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : None to poor
Colony colour : Brown-black
Lecithinase halo : Negative
Organism
Micrococcus luteus ATCC 10240
Inoculum (cfu) 10-100
Growth : Poor to good
Colony colour : Very small colony in brown black
Lecithinase halo : Negative
Applications
Used for selective isolation of cornybacteria and staphylococci
Composition
Potassium Tellurite
0.35 gm
Distilled water
10.00 ml
Directions
1. Aseptically add to sterile cooled media at 45-50°C and dispense as desired.
2. Add 0.3 ml of Potassium Tellurite 3.5% Supplement to 19 ml of Giolitti-Cantoni Broth.
3. Add 3 ml of Potassium Tellurite 3.5% Supplement to 950 ml of Baird-Parker Agar Base w/Sulpha (21748).
4. Add 10 ml of Potassium Tellurite 3.5% Supplement in 1000 ml of Hoyle Medium base(46946).
