Appearance (Colour)
Light green
Appearance (Form)
Free flowing, homogeneous powder
Solubility
8.00 gm/liter
Solubility before autoclaving (Clarity)
Clear without any precipitate
pH (25°C)
6.7 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear without any precipitate
Prepared Medium Appearance after autoclaving (Colour)
Bluish green, clear without any precipitate
Cultural Response
Inoculate and incubate at 35-37°C for 18-48 hours
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good, Malonate Utilization : (+)
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Fair - good, Malonate Utilization : (-)
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Poor - fair, Malonate Utilization : (-)
Organism
Klebsiella pneumoniae ATCC 13883
Inoculum (cfu) 10-100
Growth : Good, Malonate Utilization : (+)
Key
Malonate Utilisation :
(+) :Dark blue colour
(-) : No colour change of medium
Applications
Used for differentiating Enterobacter and Escherichia coli based on malonate utilization.
Composition
Ingredients
gm/lt.
Ammonium sulphate
2.00
Dipotassium phosphate
0.60
Monopotassium phosphate
0.40
Sodium chloride
2.00
Sodium malonate
3.00
Bromothymol Blue
0.025
Directions
1. Add 8.00 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Dispense into test tubes.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
