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Listeria Oxford Medium Base (Oxford Agar) new
Concentration  55.6 gm/lit.

Part D
HSN Code : 38210000
IMDG Identification : Not Regulated for Transport (Non-Haz)
Storage : 8 to 25°C (Cool & dry area)
Specifications
Appearance (Colour)Biege
Appearance (Form)Free flowing, homogeneous powder
Solubility55.50 gm/liter
Solubility before autoclaving (Clarity)Clear to slightly opalescent gel with a bluish tinge
Gel strengthFirm, comparable with 1.5% agar gel
pH (25°C)7.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)Clear to slightly opalescent gel with a bluish tinge
Prepared Medium Appearance after autoclaving (Colour)Dark amber
Cultural ResponseInoculate and incubate at 35 ± 2°C for 24-48 hours
OrganismListeria monocytogenes ATCC 19112
Inoculum (cfu) 10-100Growth : Good,
Recovery rate : >=50%,
Esculin hydrolysis : Positive reaction, blackening of medium around colony
OrganismStaphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100Growth : Good,
Recovery rate :>=40.0 - 50.0%,
Esculin hydrolysis : Negative reaction
OrganismEscherichia coli ATCC 25922
Inoculum>=103,Growth : Inhibited
OrganismBacillus subtilis ATCC 6633
Inoculum>=103,Growth : Inhibited
Notes
  Applications:
Used for isolation of Listeria species from pathological samples.

Composition:
Ingredients : gm/lt.
Neopeptone : 23.00
Sodium chloride : 5.00
Corn Starch : 1.00
Esculin : 1.00
Lithium chloride : 15.00
Ammonium Ferric citrate : 0.50
Agar : 10.00

Directions:
1.Add 27.75 gm powder to 500 ml distilled/deionized water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 121 °C at 15 psi pressure for 15 minutes.
4. Aseptically,add the rehydrated contents of 1 vial of Oxford Listeria Supplement (42116) or 1 vial of Listeria Moxalactam Supplement (15650).
5. Mix well and dispense.
Available Packages
100 Gms500 Gms






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Disclaimer
The information represented here may/may not represent the entire product specification, application or protocol recommended by Sisco Research Laboratories Pvt. Ltd. (SRL). This information is for the user scientists or trading community as a guide in their applications. The company claims no liability for misuse resulting due to wrong usage of the information above. For actual batch related documents, mail us.
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