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Specifications |
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Appearance (Form) | Liquid | Appearance (Colour) | Colourless | Appearance (Clarity) | Clear | Concentration | 1U/μl | Unit Definition | One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C. | Amplification capability | Upto 5kb DNA fragment | Absence of nucleases | Confirmed | DNA contamination | No Genomic or Plasmid DNA contamination |
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Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. Taq DNA Polymerase can amplify DNA target up to 5 kb (simple template). The elongation velocity is 0.9~1.2kb/min (70~75°C). It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity that results in a 3'-dA overhangs PCR product.
Applications
PCR amplification of DNA fragments as long as 5 kb
DNA labeling.
DNA sequencing.
PCR for cloning.
Advantages:
Ease of use, no dilution required.
Includes:
500 units
• TAQ Polymerase (1U/μl) - 500 μl
• 10X PCR Buffer (MgCl2 free) - 1.25ml
• 25mM MgCl2 - 1.0ml
1000 units
• TAQ Polymerase (1U/μl) - 1000 μl
• 10X PCR Buffer (MgCl2 free) - 1.25ml x 2
• 25mM MgCl2 - 1.0ml
5000 Units
• TAQ Polymerase (1U/μl) - 1000 μl x 5
• 10X PCR Buffer (MgCl2 free) - 1.25ml x 10
• 25mM MgCl2 - 1.0ml x 5 |
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Available Packages |
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Size |
Price |
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500 Units |
INR 2280.00
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Add to Cart |
1000 Units |
INR 4320.00
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Add to Cart |
5000 Units |
INR 21010.00
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Add to Cart |
Prices indicative & subject to additional charges.
(Indian Rupees (INR))
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