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Pvt. Ltd.


Pfu DNA Polymerase (5U/µl)

Part E
Storage : -20 °C (Blue/Dry Ice)
Shelf Life : 24 Months
HSN Code : 38229090
IMDG Identification :Not Regulated for Transport (Non-Haz)
Product Datasheet
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Appearance (Form)Liquid
Appearance (Colour)Colourless
Appearance (Clarity)Clear
Amplification capabilityUp to 2kb DNA fragment
Absence of nucleasesNo DNA degradation seen proving absence of nuleases
DNA contaminationNo Genomic or Plasmid DNA contamination
Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has been shown to exhibit superior thermostability and proofreading properties compared to other thermostable polymerase. Unlike Taq DNA polymerase, highly thermostable Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA polymerase in your PCR reactions results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis.

High-fidelity PCR and primer-extension reactions
PCR cloning and blunt-ended amplification product generation

250 Units
• Pfu TAQ Polymerase (5U/μl) - 50 μl
• 10X Pfu TAQ Buffer with MgCl2 - 1.25ml
• 6 X Loading Buffer - 1.0ml

1000 Units
• Pfu TAQ Polymerase (5U/μl) - 200 μl
• 10X Pfu TAQ Buffer with MgCl2 - 1.25ml x 2
• 6 X Loading Buffer - 1.0ml
Available Packages
Size Price  
250 Units
INR 6480.00  
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1000 Units
INR 22720.00  
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Prices indicative & subject to additional charges. (Indian Rupees (INR))
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  Pfu DNA Polymerase (5U/µl)  
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