srl chemicals   Sisco
Research
Laboratories
Pvt. Ltd.
Search  
 

76516

M-MLV (Recombinant)

Reverse Transcriptase
Part E
Storage : -20 °C (Blue/Dry Ice)
Shelf Life : 24 Months
HSN Code : 38229090
IMDG Identification :Not Regulated for Transport (Non-Haz)
 
Product Datasheet
Stock Check & Taxation Details
Enter Batch No. for COA
View Sample COA
 
Specifications
 
Appearance (Form)Liquid
Appearance (Colour)Colourless
Appearance (Clarity)Clear
Absence of nucleasesTested using relevant procedures
Amplification capabilityTested by RT-PCR
Notes
  Description:
Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) uses single-stranded RNA or DNA in the presence of a primer to synthesize a complementary DNA
strand.

This enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. The enzyme is used to synthesize first-strand cDNA up to 5 kb.

The enzyme has increased thermal stability, that allows the reaction to be carried out at a higher temperature (optimum activity at 50°C). It increases the efficiency and specificity of those transcribed RNA regions which are rich in GC pairs and/or contain secondary structures. The enzyme has no 3’ to 5’ exonuclease and reduced RNaseH activity, that improves the synthesis of a full-length cDNA, even from long mRNA templates, using random priming. The enzyme gives high yields of first strand cDNa synthesis up to 10kb long.

Applications:
PCR,
real-time PCR
second strand cDNA synthesis
DNA labeling
Analysis of RNA by primer extension

Includes:
5000U
· M-MLV (Recombinant) (200U/μl ) - 25 μl
· Reaction Buffer (10X) - 50 μl

10000U
· M-MLV (Recombinant) (200U/μl ) - 50 μl
· Reaction Buffer (10X) - 100 μl
 
Available Packages
 
Size Price  
5000 Units
INR 9000.00  
  Add to Cart
10000 Units
INR 16000.00  
  Add to Cart
Prices indicative & subject to additional charges. (Indian Rupees (INR))
 
Customers Also Viewed
 
  41066
TRIzol-C Reagent
  60159
BioLit FluroGreen qPCR Master Mix (2X)
  71969
RNA Stabilizing Solution