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47167

KpnI (10U/µl)


Part E
Storage : -20 °C (Blue/Dry Ice)
Shelf Life : 24 Months
HSN Code : 38229090
IMDG Identification :Not Regulated for Transport (Non-Haz)
 
Product Datasheet
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Specifications
 
Appearance (Form) Liquid
Appearance (Colour)Colourless
Appearance (Clarity)Clear
ActivityOne unit is defined as the amount of enzyme required to digest 1 μg of lambda DNA in 1 hour at 37 °C in 50 μL of assay buffer
DNA degradationIncubation of single stranded and double stranded radiolabeled oligonucleotides with 10 units of the enzyme for 4 hours at 37 °C
Star AcitvityAfter 160-fold overdigestion (10 U/μg Lambda DNA for 16 hours at 37 °C) with enzyme the fragmentation pattern is analysed. No detaectable changes compared to the theoretical fragmentation pattern are considered as absence of star activity
Notes
  Description:
KpnI is an endonuclease enzyme isolated from strains of Klebsiella pneumoniae Ok8, and is part of the restriction modification system.
In molecular biology it is used as a restriction enzyme. It creates sticky ends with 3' end overhangs. The nucleic acid sequence where the enzyme cuts is GGTACC, which, as the complementary sequence is CCATGG, has rotational symmetry.

Includes:
1000U
• KpnI - 100 μl
• 10X Reaction Buffer - 1.0ml
2000U
• KpnI - 200 μl
• 10X Reaction Buffer - 1.0ml
 
Available Packages
 
Size Price  
1000 Units
INR 2730.00  
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2000 Units
INR 5140.00  
  Add to Cart
Prices indicative & subject to additional charges. (Indian Rupees (INR))
 
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