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99359

TRIzol-T Reagent


Part E
Storage : 2 to 8°C (Refrigerate)
Shelf Life : 12 Months
HSN Code : 38229090
IMDG Identification :Not Regulated for Transport (Non-Haz)
 
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Specifications
 
Appearance (Form)Liquid
Appearance (Colour)Transparent to Straw/pale Yellow
Agarose gel electrophoresisShows clear bands of RNA
RNA isolationRNA bands of good intensity
StabilityStable for one year from the date of manufacture
Notes
  Description:
TRIzol-T Reagent is a ready-to-use reagent for the isolation of total RNA from cells and tissues. The reagent, a mono-phasic solution of phenol and guanidine isothiocyanate, is an improvement to the single-step RNA isolation method and gives high yields of Total RNA.
During sample homogenization or lysis, TRIzol-T Reagent maintains the integrity of the RNA, while disrupting cells and dissolving cell components. Addition of chloroform followed by centrifugation, separates the solution into an aqueous phase and an organic phase. RNA remains exclusively in the aqueous phase. After transfer of the aqueous phase, the RNA is recovered by precipitation with isopropyl alcohol. After removal of the aqueous phase, the DNA and proteins in the sample can be recovered by sequential precipitation. Precipitation with ethanol yields DNA from the interphase, and an additional precipitation with isopropyl alcohol yields proteins from the organic phase. Copurification of the DNA may be useful for normalizing RNA yields from sample to sample.This technique performs well with small quantities of tissue (50-100 mg) and cells (5 × 106), and large quantities of tissue (≥1 g) and cells (>107), of human, animal, plant, or bacterial origin.

Applications:
It can be used for Northern blot analysis, dot blot hybridization,poly (A)+ selection, in vitro translation, RNase protection assay, and molecular cloning.


 
Available Packages
 
Size Price  
20 ml
INR 1600.00  
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100 ml
INR 6140.00  
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Prices indicative & subject to additional charges. (Indian Rupees (INR))
 
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  TRIzol-T Reagent  
 
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