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| Specifications |
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| Appearance (Form) | Liquid | | Appearance (Colour) | Pale Yellow (Straw coloured) | | Agarose gel electrophoresis | Clear bands of RNA seen | | RNA isolation | RNA Bands of good intensity | | Stability | Stable for on year from the date of manufacture |
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Description:
TRIzol-C Reagent is our new and cost-effective ready-to-use formulation for isolation of pure total RNA from cells and tissues. The reagent, a mono-phasic solution of phenol and guanidine isothiocyanate, is an improvement giving very high yields of pure RNA useful for downstream processes .
During sample homogenization or lysis, TRIzol-C Reagent maintains the integrity of the RNA, while disrupting cells and dissolving cell components. Addition of chloroform followed by centrifugation, separates the solution into an aqueous phase and an organic phase. RNA remains exclusively in the aqueous phase. After transfer of the aqueous phase, the RNA is recovered by precipitation with isopropyl alcohol. After removal of the aqueous phase, the DNA and proteins in the sample can be recovered by sequential precipitation. Precipitation with ethanol yields DNA from the interphase, and an additional precipitation with isopropyl alcohol yields proteins from the organic phase. Copurification of the DNA may be useful for normalizing RNA yields from sample to sample.
Applications:
It can be used for Northern blot analysis, dot blot hybridization,poly (A)+ selection, in vitro translation, RNase protection assay, and molecular cloning. |
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| Available Packages |
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| Size |
Price |
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| 20 ml |
INR 1820.00
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Add to Cart |
| 100 ml |
INR 6360.00
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Add to Cart |
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Prices indicative & subject to additional charges.
(Indian Rupees (INR))
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![TRIzol-C Reagent](javascript:showProductImage("%2Fuser_img%2Fproducts%2F41066%2F41066-TRIzol-C%20Reagent.jpg") "TRIzol-C Reagent")