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26221

Hugh Leifson Medium (B/S)


Part D
Storage : 8 to 25°C (Cool & dry area)
Shelf Life : 36 Months
HSN Code : 38210000
IMDG Identification :Not Regulated for Transport (Non-Haz)
 
MSDS
Product Datasheet
Stock Check & Taxation Details
Enter Batch No. for COA
 
Specifications
 
Appearance (Clarity)Light yellow with bluish green tint
Appearance (Colour)Free flowing, homogeneous powder
Solubility20.33 gm/liter
Solubility before autoclaving (Clarity)Clear to very slightly opalescent
pH (25°C)7.1 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)Greenish blue
Cultural ResponseInoculate and incubate at 37° C for 18 - 48 hours
OrganismEnterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100Growth:Good
Motility : Positive
Aerobic Fermentation : Acid(yellow), Gas production : Positive
Anaerobic Fermentation : Acid(yellow), Gas production : Positive
OrganismEscherichia coli ATCC 25922
Inoculum (cfu) 10-100Growth : Good
Motility : Positive
Aerobic Fermentation : Acid(yellow), Gas production : Positive
Anaerobic Fermentation : Acid(yellow), Gas production : Positive
OrganismPseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100Growth:Good
Motility : Positive
Aerobic Fermentation : Acid(yellow), Gas production : Positive
Anaerobic Fermentation : Green/Blue, Gas production : Negative
OrganismSalmonella typhimurium ATCC 6539
Inoculum (cfu) 10-100Growth:Good
Motility : Positive
Aerobic Fermentation : Acid(yellow), Gas production : Positive
Anaerobic Fermentation : Acid(yellow), Gas production : Positive
Notes
  Applications:
Used to distinguish between anaerobic and aerobic breakdown of carbohydrate (Dextrose).

Composition:
Ingredients : gm/lt.
Peptone : 2.00
Sodium chloride : 5.00
Dipotassium phosphate : 0.30
Dextrose : 10.0
Bromothymol Blue : 0.03
Agar : 3.00

Directions:
1. Add 20.33 gm powder to 1.0 liter of distilled/deionized water and mix thoroughly.
2. Gently heat and bring to boiling
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
 
Available Packages
 
Size Price  
100 Gms
INR 900.00  
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500 Gms
INR 3860.00  
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Prices indicative & subject to additional charges. (Indian Rupees (INR))
 
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