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38080

Ribonuclease H (RNase H) (5U/ul)


Part E
Storage : -20 °C (Blue/Dry Ice)
Shelf Life : 24 Months
HSN Code : 35079099
IMDG Identification :Not Regulated for Transport (Non-Haz)
 
Product Datasheet
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Specifications
 
Appearance (Form)Liquid
Appearance (Colour)Colourless
Appearance (Clarity)Clear
DNase activityNone detected
Protease activityNone detected
Concentration5U/μl
Notes
  Description:
RNase H is a 18.9 kDa recombinant endoribonuclease purified from an Escherichia coli strain, which over-expresses cloned RNase H gene (rnh). The enzyme hydrolyses specifically the phosphodiester bonds of RNA hybridized to DNA and produces 5´ phosphate-terminated oligoribonucleotides and single-stranded DNA.
Rnase H does not degrade single and double-stranded DNA or unhybridized RNA. It is a key enzyme in the removal of mRNA after first-strand cDNA synthesis. Treating cDNA with RNase H prior to PCR can improve sensitivity as RNA bonded to the cDNA template may prevent binding of the amplification primers in a PCR reaction. RNase H treatment is often necessary when amplifying longer, full-length cDNA targets. In addition, RNase H is useful for the removal of poly(A) tails on mRNAs after hybridization with oligo(dT) and also for the site-specific enzymatic cleavage of RNA.

Includes:

50U
RNAse H (5U/µl) - 10µl
10 X RNase H Reaction Buffer - 40 µl

250U
RNAse H (5U/µl) - 50µl
10 X RNase H Reaction Buffer - 200 µl

Applications:
Removal of RNA after first strand cDNA synthesis (RT-PCR and qRT-PCR)
Removal of mRNA prior to synthesis of second strand cDNA
Removal of the poly(A) sequences of mRNA after hybridization with oligo(dT)
Site-specific cleavage of RNA
Studies of in vitro polyadenylation reaction products
 
Available Packages
 
Size Price  
50 Units
INR 7580.00  
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250 Units
INR 22720.00  
  Add to Cart
Prices indicative & subject to additional charges. (Indian Rupees (INR))
 
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