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| Specifications |
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| Appearance (Form) | Liquid | | Appearance (Colour) | Colourless | | Appearance (Clarity) | Clear | | DNase activity | None detected | | Protease activity | None detected | | Concentration | 5U/μl |
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Description:
RNase H is a 18.9 kDa recombinant endoribonuclease purified from an Escherichia coli strain, which over-expresses cloned RNase H gene (rnh). The enzyme hydrolyses specifically the phosphodiester bonds of RNA hybridized to DNA and produces 5´ phosphate-terminated oligoribonucleotides and single-stranded DNA.
Rnase H does not degrade single and double-stranded DNA or unhybridized RNA. It is a key enzyme in the removal of mRNA after first-strand cDNA synthesis. Treating cDNA with RNase H prior to PCR can improve sensitivity as RNA bonded to the cDNA template may prevent binding of the amplification primers in a PCR reaction. RNase H treatment is often necessary when amplifying longer, full-length cDNA targets. In addition, RNase H is useful for the removal of poly(A) tails on mRNAs after hybridization with oligo(dT) and also for the site-specific enzymatic cleavage of RNA.
Includes:
50U
RNAse H (5U/µl) - 10µl
10 X RNase H Reaction Buffer - 40 µl
250U
RNAse H (5U/µl) - 50µl
10 X RNase H Reaction Buffer - 200 µl
Applications:
Removal of RNA after first strand cDNA synthesis (RT-PCR and qRT-PCR)
Removal of mRNA prior to synthesis of second strand cDNA
Removal of the poly(A) sequences of mRNA after hybridization with oligo(dT)
Site-specific cleavage of RNA
Studies of in vitro polyadenylation reaction products
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| Available Packages |
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| Size |
Price |
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| 50 Units |
INR 7580.00
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Add to Cart |
| 250 Units |
INR 22720.00
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Add to Cart |
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Prices indicative & subject to additional charges.
(Indian Rupees (INR))
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