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44521

Thayer-Martin Medium, Base

(Thayer-Martin Agar, Modified)
Concentration 42 gm/lit.
Part D
Storage : 8 to 25°C (Cool & dry area)
Shelf Life : 36 Months
HSN Code : 38210000
IMDG Identification :Not Regulated for Transport (Non-Haz)
 
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Product Datasheet
Stock Check & Taxation Details
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Specifications
 
Composition:
Agar:13.00 gm/lit.
Special Peptone:23.00 gm/lit.
NaCl:5.00 gm/lit.
Corn starch:1.00 gm/lit.
CNVT inhibitor: -
Supplement solution: -

pH at 25 °C :7.0 ± 0.2

CNVT Inhibitor:
(for 1.0 liter of medium) :
Colistin methane sulphonate:7.5 mg
Trimethoprim:5.0 mg
Vancomycin:3.0 mg
Nystatin:12,500 U
D/W: up to 10.0 ml

Vitamin Growth Supplement:
(for 1.0 liter of medium) :
Part I:
Glucose: 2 gm
Cysteine-HCl:518 mg
Cocarboxylase:2 mg
L-Glutamine:200 mg
L-Cystine:22 mg
Adenine sulphate:20 mg
NAD (Coenzyme I):50 mg
Vitamin B12:0.20 mg
Thiamine Hydrochloride:0.06 mg
Guanine-HCl:0.6 mg
Ferric nitrate:0.4 mg
p-Aminobenzoic acid:0.26 mg

Part II : Rehydrating Fluid :
Rehydrating Fluid:
Dextrose:2 gm
D/W:20 ml
Dissolve contents of Part I in 20 ml of Rehydrating Fluid.

Storage:Store between 8-25 °C.
Notes
  Organisms :
1.Neisseria gonorrhoeae ATCC 19424
2.Neisseria meningitidis ATCC 13090
3.Escherichia coli ATCC 25922
4.Proteus mirabilis ATCC 25933

Directions :
1.Add 42.0 gm powder to distilled/deionized water.
2.Bring volume to 900.0 ml and mix thoroughly.
3.Gently heat and bring to boiling.
4.Autoclave at 15 psi pressure at 121°C for 15 minutes.
5.Cool to 450-50°C and aseptically add 100 ml of sterile lysed blood,10.0 ml of sterile CNVT inhibitor and 20.0 ml of Vitamin Growth Supplement. Mix well.

Applications :
For the isolation of Neisseria species from specimens containing mixed flora of bacteria and fungi.
 
Available Packages
 
Size Price  
100 Gms
INR 760.00  
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500 Gms
INR 3300.00  
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Prices indicative & subject to additional charges. (Indian Rupees (INR))
 
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